Πλοήγηση ανά Θέμα "next generation sequencing"
Αποτελέσματα 1-17 από 17
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Aspects of hereditary angioedema genotyping in the era of NGS: The case of F12 gene [Wybrane aspekty genotypowania wrodzonego obrzȩku naczynioruchowego w erze NGS: Gen F12]
(2018)Objective. To screen a cohort of patients diagnosed with non-FXII angioedema for carriage of variants of F12 gene. Material and methods. DNA samples from 191 patients suffering from primary angioedema with normal C1-INH, ... -
Assessment of the effects of oxamyl on the bacterial community of an agricultural soil exhibiting enhanced biodegradation
(2019)Modern agricultural practices largely rely on pesticides to protect crops against various pests and to ensure high yields. Following their application to crops a large amount of pesticides ends up in soil where they may ... -
The fate of BRCA1-related germline mutations in triple-negative breast tumors
(2017)The preservation of pathogenic BRCA1/2 germline mutations in tumor tissues is usually not questioned, while it remains unknown whether these interact with somatic genotypes for patient outcome. Herein we compared germline ... -
Functional analysis of miRNAs using the DIANA tools online suite
(2017)microRNAs (miRNAs) are central regulators of gene expression. They are actively studied for their involvement in numerous physiological and pathological conditions but also as diagnostic biomarkers or promising therapeutic ... -
Lab to field assessment of the ecotoxicological impact of chlorpyrifos, isoproturon, or tebuconazole on the diversity and composition of the soil bacterial community
(2018)Pesticides are intentionally applied to agricultural fields for crop protection. They can harm non-target organisms such as soil microorganisms involved in important ecosystem functions with impacts at the global scale. ... -
LaGomiCs - Lagomorph Genomics Consortium: An International Collaborative Effort for Sequencing the Genomes of an Entire Mammalian Order
(2016)The order Lagomorpha comprises about 90 living species, divided in 2 families: the pikas (Family Ochotonidae), and the rabbits, hares, and jackrabbits (Family Leporidae). Lagomorphs are important economically and scientifically ... -
A meta-barcoding approach to assess and compare the storage temperature-dependent bacterial diversity of gilt-head sea bream (Sparus aurata) originating from fish farms from two geographically distinct areas of Greece
(2018)Bacterial diversity of whole gilt-head sea bream (Sparus aurata L. 1758) originating from Ionian and Aegean Sea aquaculture farms and stored at 0 (ice), 4 and 8 °C was determined by 16S rRNA gene amplicon sequencing method ... -
Mitogenomic analysis in European brown hare (Lepus europaeus) proposes genetic and functional differentiation between the distinct lineages
(2018)European brown hare is a small game species spreading across Europe to Asia Minor, with important economic traits. Population genetics studies using mitochondrial DNA markers have revealed the existence of two major ... -
Molecular approaches for HPV genotyping and HPV-DNA physical status
(2017)Persistent infection with high-risk human papillomavirus (HPV) genotypes is the leading cause of cervical cancer development. To this end several studies have focused on designing molecular assays for HPV genotyping, which ... -
Mussel biofiltration effects on attached bacteria and unicellular eukaryotes in fish-rearing seawater
(2016)Mussel biofiltration is a widely used approach for the mitigation of aquaculture water. In this study, we investigated the effect of mussel biofiltration on the communities of particle-associated bacteria and unicellular ... -
Prognostic significance of tumor genotypes and CD8+ infiltrates in stage I-III colorectal cancer
(2018)Background: We explored the clinical significance of tumor genotypes and immunophenotypes in non-metastatic colorectal cancer (CRC). Methods: In primary tumors (paraffin blocks) from 412 CRC patients treated with adjuvant ... -
Screening for TSC1 and TSC2 mutations using NGS in Greek children with tuberous sclerosis syndrome
(2018)Tuberous Sclerosis Complex (TSC) is a rare neurocutaneous syndrome inherited by an autosomal dominant manner. The disorder is commonly manifested by the presence of multiple benign tumors located in numerous tissues, ... -
Targeted next-generation sequencing for the molecular diagnosis of hereditary angioedema due to C1-inhibitor deficiency
(2018)SERPING1 genotyping of subjects suspicious for hereditary angioedema due to C1-INH deficiency (C1-INH-HAE) is important for clinical practice as well as for research reasons. Conventional approaches towards the detection ... -
Thumb duplication: molecular analysis of different clinical types
(2019)Purpose: Molecular analysis of different types of thumb duplication and identification of new suspected gene mutations. Materials and methods: In a series of patients operated for polydactyly, DNA was extracted from blood ... -
Tumor heterogenicity: Multiple needle biopsies from different lesion sites-key to successful targeted therapy and immunotherapy
(2018)[No abstract available] -
Variability of airborne bacteria in an urban Mediterranean area (Thessaloniki, Greece)
(2017)The abundance, biomass and the taxonomic composition of the total airborne bacterial communities in a coastal urban area of Northeastern Mediterranean Sea were examined. In total, 27 air samples were collected across three ... -
Viruses and Multiple Sclerosis: From Mechanisms and Pathways to Translational Research Opportunities
(2017)Viruses are directly or indirectly implicated in multiple sclerosis (MS). Here, we review the evidence on the virus-related pathophysiology of MS, introduce common experimental models, and explore the ways in which viruses ...