Πλοήγηση ανά Θέμα "binding site"

Affinity Crystallography Reveals Binding of Pomegranate Juice Anthocyanins at the Inhibitor Site of Glycogen Phosphorylase: The Contribution of a Sugar Moiety to Potency and Its Implications to the Binding Mode

Drakou C.E., Gardeli C., Tsialtas I., Alexopoulos S., Mallouchos A., Koulas S.M., Tsagkarakou A.S., Asimakopoulos D., Leonidas D.D., Psarra A.-M.G., Skamnaki V.T. (2020)

Anthocyanins (ACNs) are dietary phytochemicals with an acknowledged therapeutic significance. Pomegranate juice (PJ) is a rich source of ACNs with potential applications in nutraceutical development. Glycogen phosphorylase ...

Amino acid signatures in the HLA class II peptide-binding region associated with protection/susceptibility to the severe West Nile Virus disease

Sarri C.A., Papadopoulos G.E., Papa A., Tsakris A., Pervanidou D., Baka A., Politis C., Billinis C., Hadjichristodoulou C., Mamuris Z. (2018)

The MHC class II region in humans is highly polymorphic. Each MHC molecule is formed by an α and a β chain, produced by different genes, creating an antigen-binding groove. In the groove there are several pockets into which ...

The ammonium sulfate inhibition of human angiogenin

Chatzileontiadou D.S.M., Tsirkone V.G., Dossi K., Kassouni A.G., Liggri P.G.V., Kantsadi A.L., Stravodimos G.A., Balatsos N.A.A., Skamnaki V.T., Leonidas D.D. (2016)

In this study, we investigate the inhibition of human angiogenin by ammonium sulfate. The inhibitory potency of ammonium sulfate for human angiogenin (IC50 = 123.5 ± 14.9 mm) is comparable to that previously reported for ...

Aqueous Solubility Enhancement for Bioassays of Insoluble Inhibitors and QSPR Analysis: A TNF-α Study

Mettou A., Papaneophytou C., Melagraki G., Maranti A., Liepouri F., Alexiou P., Papakyriakou A., Couladouros E., Eliopoulos E., Afantitis A., Kontopidis G. (2018)

The aim of this study is to improve the aqueous solubility of a group of compounds without interfering with their bioassay as well as to create a relevant prediction model. A series of 55 potential small-molecule inhibitors ...

Assessment of remediation of soils, moderately contaminated by potentially toxic metals, using different forms of carbon (charcoal, biochar, activated carbon). Impacts on contamination, metals availability and soil indices

Golia E.E., Aslanidis P.-S.C., Papadimou S.G., Kantzou O.-D., Chartodiplomenou M.-A., Lakiotis K., Androudi M., Tsiropoulos N.G. (2022)

The present study aims to assess the effect of three forms of carbon, i.e., charcoal, biochar and activated carbon using as soil amendments (or soil conditioners) in two soils, an acid and alkaline one. For this purpose, ...

AtHESPERIN: A novel regulator of circadian rhythms with poly(A)-degrading activity in plants

Delis C., Krokida A., Tomatsidou A., Tsikou D., Beta R.A.A., Tsioumpekou M., Moustaka J., Stravodimos G., Leonidas D.D., Balatsos N.A.A., Papadopoulou K.K. (2016)

We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the ...

DIANA-LncBase v2: Indexing microRNA targets on non-coding transcripts

Paraskevopoulou M.D., Vlachos I.S., Karagkouni D., Georgakilas G., Kanellos I., Vergoulis T., Zagganas K., Tsanakas P., Floros E., Dalamagas T., Hatzigeorgiou A.G. (2016)

MicroRNAs (miRNAs) are short non-coding RNAs (ncRNAs) that act as post-transcriptional regulators of coding gene expression. Long non-coding RNAs (lncRNAs) have been recently reported to interact with miRNAs. The sponge-like ...

DIANA-mirExTra v2.0: Uncovering microRNAs and transcription factors with crucial roles in NGS expression data

Vlachos I.S., Vergoulis T., Paraskevopoulou M.D., Lykokanellos F., Georgakilas G., Georgiou P., Chatzopoulos S., Karagkouni D., Christodoulou F., Dalamagas T., Hatzigeorgiou A.G. (2016)

Differential expression analysis (DEA) is one of the main instruments utilized for revealing molecular mechanisms in pathological and physiological conditions. DIANA-mirExTra v2.0 (http://www.microrna. gr/mirextrav2) ...

DIANA-miRGen v3.0: Accurate characterization of microRNA promoters and their regulators

Georgakilas G., Vlachos I.S., Zagganas K., Vergoulis T., Paraskevopoulou M.D., Kanellos I., Tsanakas P., Dellis D., Fevgas A., Dalamagas T., Hatzigeorgiou A.G. (2016)

MicroRNAs (miRNAs) are small non-coding RNAs that actively fine-tune gene expression. The accurate characterization of the mechanisms underlying miRNA transcription regulation will further expand our knowledge regarding ...

DIANA-miRGen v4: Indexing promoters and regulators for more than 1500 microRNAs

Perdikopanis N., Georgakilas G.K., Grigoriadis D., Pierros V., Kavakiotis I., Alexiou P., Hatzigeorgiou A. (2021)

Deregulation of microRNA (miRNA) expression plays a critical role in the transition from a physiological to a pathological state. The accurate miRNA promoter identification in multiple cell types is a fundamental endeavor ...

DIANA-miTED: A microRNA tissue expression database

Kavakiotis I., Alexiou A., Tastsoglou S., Vlachos I.S., Hatzigeorgiou A.G. (2022)

microRNAs (miRNAs) are short (∼23nt) single-stranded non-coding RNAs that act as potent post-transcriptional gene expression regulators. Information about miRNA expression and distribution across cell types and tissues is ...

DIANA-tarbase and DIANA suite tools: Studying experimentally supported microRNA targets

Paraskevopoulou M.D., Vlachos I.S., Hatzigeorgiou A.G. (2016)

microRNAs (miRNAs) are short non-coding RNAs (~22 nts) present in animals, plants, and viruses. They are considered central post-transcriptional regulators of gene expression and are key components in a great number of ...

Discovery and characterization of 2-anilino-4- (thiazol-5-yl)pyrimidine transcriptional CDK inhibitors as anticancer agents

Wang, S.; Griffiths, G.; Midgley, C. A.; Barnett, A. L.; Cooper, M.; Grabarek, J.; Ingram, L.; Jackson, W.; Kontopidis, G.; McClue, S. J.; McInnes, C.; McLachlan, J.; Meades, C.; Mezna, M.; Stuart, I.; Thomas, M. P.; Zheleva, D. I.; Lane, D. P.; Jackson, R. C.; Glover, D. M.; Blake, D. G.; Fischer, P. M. (2010)

The main difficulty in the development of ATP antagonist kinase inhibitors is target specificity, since the ATP-binding motif is present in many proteins. We introduce a strategy that has allowed us to identify compounds ...

ERK signaling controls productive HIF-1 binding to chromatin and cancer cell adaptation to hypoxia through HIF-1α interaction with NPM1

Koukoulas K., Giakountis A., Karagiota A., Samiotaki M., Panayotou G., Simos G., Mylonis I. (2021)

The hypoxia-inducible factor HIF-1 is essential for oxygen homeostasis. Despite its well-understood oxygen-dependent expression, regulation of its transcriptional activity remains unclear. We show that phosphorylation by ...

Estimating the total number of phosphoproteins and phosphorylation sites in eukaryotic proteomes

Vlastaridis P., Kyriakidou P., Chaliotis A., Van de Peer Y., Oliver S.G., Amoutzias G.D. (2017)

Background: Phosphorylation is the most frequent post-translational modification made to proteins and may regulate protein activity as either a molecular digital switch or a rheostat. Despite the cornucopia of high-throughput ...

An evaluation of indirubin analogues as phosphorylase kinase inhibitors

Begum, J.; Skamnaki, V. T.; Moffatt, C.; Bischler, N.; Sarrou, J.; Skaltsounis, A. L.; Leonidas, D. D.; Oikonomakos, N. G.; Hayes, J. M. (2015)

Phosphorylase kinase (PhK) has been linked with a number of conditions such as glycogen storage diseases, psoriasis, type 2 diabetes and more recently, cancer (Camus et al., 2012 [6]). However, with few reported structural ...

Evidence for Novel Action at the Cell-Binding Site of Human Angiogenin Revealed by Heteronuclear NMR Spectroscopy, in silico and in vivo Studies

Chatzileontiadou D.S.M., Tsika A.C., Diamantopoulou Z., Delbé J., Badet J., Courty J., Skamnaki V.T., Parmenopoulou V., Komiotis D., Hayes J.M., Spyroulias G.A., Leonidas D.D. (2018)

A member of the ribonuclease A superfamily, human angiogenin (hAng) is a potent angiogenic factor. Heteronuclear NMR spectroscopy combined with induced-fit docking revealed a dual binding mode for the most antiangiogenic ...

Functional analysis of miRNAs using the DIANA tools online suite

Vlachos I.S., Hatzigeorgiou A.G. (2017)

microRNAs (miRNAs) are central regulators of gene expression. They are actively studied for their involvement in numerous physiological and pathological conditions but also as diagnostic biomarkers or promising therapeutic ...

Functional characterization and anti-cancer action of the clinical phase II cardiac Na+/K+ ATPase inhibitor istaroxime: In vitro and in vivo properties and cross talk with the membrane androgen receptor

Alevizopoulos K., Dimas K., Papadopoulou N., Schmidt E.-M., Tsapara A., Alkahtani S., Honisch S., Prousis K.C., Alarifi S., Calogeropoulou T., Lang F., Stournaras C. (2016)

Sodium potassium pump (Na+/K+ ATPase) is a validated pharmacological target for the treatment of various cardiac conditions. Recent published data with Na+/K+ ATPase inhibitors suggest a potent anti-cancer action of these ...

Genetic polymorphism of canine cytochrome P450 2D25 gene

Pappas, I. S.; Katsiabas, D. S. (2004)

Human CYP2D6 is involved in the metabolism of a number of drugs including neuroleptics, tricyclic antidepressants, β-adrenoreceptors antagonists and antiarrhythmic drugs. The corresponding canine CYP2D15 have shown similar ...