Πλοήγηση ανά Θέμα "PCR"
Αποτελέσματα 21-40 από 47
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Evaluation of lymph node and bone marrow cytology in the diagnosis of canine leishmaniasis (Leishmania infantum) in symptomatic and asymptomatic
(2005)The sensitivity and specificity of lymph node and bone marrow smear microscopy for the diagnosis of Leishmania infantum-infected dogs was evaluated in 79 dogs with leishmaniasis (Group A), 52 asymptomatically infected dos ... -
Evolution of European Cockchafers (Melolonthinae: Scarabaeidae: Coleoptera): a morphological, molecular and chromosomal study of intra- and inter-specific variations
(2011)In cockchafers of the genus Melolontha, there is a marked intraspecific polymorphism for morphological characters, making some specimens of one species resemble another. A cytogenetic and molecular (mitochondrial COI gene ... -
fbl Gene as a Species-Specific Target for Staphylococcus lugdunensis Identification
(2010)Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis ... -
Field Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece
(2015)Greece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme ... -
Genetic variation in populations of the endangered fish Ladigesocypris ghigii and its implications for conservation
(2005)1. The genetic variation of the endangered freshwater fish Ladigesocypris ghigii, endemic to the island of Rhodes (Greece), was investigated for nine populations, originating from seven different stream systems and a ... -
High-Resolution Melting approaches towards plant fungal molecular diagnostics
(2015)Reliable and early molecular detection of phytopathogenic fungi is crucial in an era where the expansion of global trade in plant material is undoubtedly increasing the risk of invasive outbreaks, with devastating effects ... -
Identification of multi-resistant strains of Acinetobacter baumannii from an ICU in Greece using sequence-based typing and PFGE
(2011)Acinetobacter baumannii has emerged as an important nosocomial pathogen in many European countries, including Greece. In this study, we analysed 20 multi-resistant A. baumannii strains, derived from the First intensive ... -
Increasing prevalence of high-level gentamicin resistance among enterococci isolated in Greece
(2001)High-level gentamicin-resistant enterococci (HLGRE) (MIC >512 mug/ml) were frequently recovered in the AHEPA University Hospital, Thessaloniki, Greece, during a 15-month period (67/158, 42.4%). PCR testing showed that ... -
Leptospirosis: An important re-emerging infection of animals and man
(2010)Leptospirosis, a re-emerging infection of animals and man, is caused by one of 200 serotypes of Leptospira spp. The genus is currently divided into eight pathogenic species, infecting various animal species and man, either ... -
Level of Streptococcus pyogenes in patients with recurrent tonsillitis and tonsillar hypertrophy
(2008)Recurrent tonsillitis is 1 of the common human infectious diseases worldwide, but, to date, its pathogenesis remains unclear. Although Streptococcus pyogenes (GAS) is involved in recurrent bouts of acute tonsillitis, ... -
Microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16S rRNA gene analysis
(2015)BACKGROUNDSea bream fillets are one of the most important value-added products of the seafood market. Fresh seafood spoils mainly owing to bacterial action. In this study an exploration of initial and spoilage microbiota ... -
A modified method for the detection of differentially expressed mRNAs without using radioactivity
(2001)We present here a modification of the original differential display approach using a single oligo(dT) primer for the reverse transcription reaction (instead of the various oligo(dT)NM primers that subdivide the pool of ... -
Molecular analysis of transferrin receptor mRNA expression in acute myeloid leukaemia
(2001)Transferrin receptor (TfR, CD71) is an integral membrane glycoprotein that mediates cellular uptake of iron. In most tissues, TfR expression is correlated positively with proliferation and regulated at the post-transcriptional ... -
Molecular characterization of wild-type polioviruses isolated in Greece during the 1996 outbreak in Albania
(2006)During the present study three type 1 poliovirus strains isolated in Greece during the 1996 poliomyelitis outbreak in Albania were retrospectively investigated and determination of their relationship with other epidemic ... -
Molecular detection of the entomopathogenic bacterium Pseudomonas entomophila using PCR
(2010)Aims: To develop a specific, fast and simple molecular method useful to detect the entomopathogenic bacterium Pseudomonas entomophila. Methods and Results: The use of bioinformatics tools allowed the identification of ... -
Mycoplasma pneumoniae and Chlamydophila pneumoniae: a comparative study in patients with nasal polyposis and healthy controls
(2015)Introduction: The role played by Mycoplasma pneumoniae and Chlamydophila pneumoniae in the pathogenesis of chronic rhinosinusitis with nasal polyps has been the object of ongoing debate. We used real-time polymerase chain ... -
Plasminogen activator activity in the porcine oviduct during the oestrous cycle
(2005)The mammalian oviduct is a dynamic tissue, which lies under the influence of ovarian steroids and produces proteins that affect various stages of fertilization and post-fertilization events. In this study, expression of ... -
Proteins from fish eggs that protect DNA from acid precipitation and inhibit DNA synthesis
(2001)We partially characterized proteins that inhibit DNA acid precipitation from various fish eggs (Sparus aurata, Dicentrarchus labrax, Mugil cephalus and Zeus faber). The active proteins were purified by acetone fractionation. ... -
Qualitative and quantitative detection of protein and genetic traits in genetically modified food
(2004)Due to the market introduction of genetically modified organisms (GMOs) in crops, foods, and ingredients, legislation worldwide came face to face with the question of the use and labeling requirements on GMO crops and their ... -
Quantitative HBsAg and HDV-RNA levels in chronic delta hepatitis
(2010)Background: Hepatitis delta virus (HDV) causes severe liver disease. Aims: To investigate the quantitative HDV-RNA, HBsAg and hepatitis B virus (HBV)DNA levels in correlation to histological, biochemical and demographical ...