Formation of a secondary corpus luteum after ultrasound-guided follicular aspiration in cows
Συγγραφέας
Amiridis, G. S.; Robertson, L.; Jeffcoate, I. A.; Belibasaki, S.; Boyd, J. S.; O'Shaughnessy, P. J.Ημερομηνία
2000Λέξη-κλειδί
Επιτομή
This paper reports the observed formation of a secondary corpus luteum (CL) in the presence of the cyclic corpus luteum, on the ovaries of a cow after ultrasound-guided follicular aspiration for oocyte recovery. The secondary structure, although smaller and lighter (4.97g vs. 6.02g) than the natm al one, had the typical macroscopic appearance of a corpus luteum. Histological examination of the structure using electron microscopy revealed typical structural features of a natural CL. Mean tissue progesterone concentration was significantly lower in the secondary CL (31.15 +/- 3.11 compared with 58.29 +/- 6.32 mu g/g tissue of the cyclic CL) and oestradiol-17 beta significantly higher than in the natural CL (108 +/- 11.6 compared with 74.2 +/- 7.81 pg/g tissue). P450(scc) and P450(17 alpha) mRNA was detected in both structures while P450(arom) and full-length mRNA FSH receptor were detected only in the secondary structure.