Nitrogen effect on growth-related parameters and evaluation of Portulaca oleracea as a phytoremediation species in a Cr(VI)-spiked soil

Abstract

In a pot experiment, we assessed the potential of purslane (Portulaca oleracea) as a phytoremediation species in Cr(VI)-contaminated soils. We focused on the evaluation of phytotoxic Cr(VI) effects at concentrations reaching 150 mg Cr(VI) kg-1 and the possible stress amelioration effect of nitrogen on Cr(VI)-stressed plants. Treatments were T-0 (control), T-1 (25 mg Cr(VI) kg-1), T-2 = 50 mg kg-1, T-3 = 100 mg kg-1, and T-4 = 150 mg kg-1. We measured Cr(VI) concentration in aerial and root tissues, a series of parameters related to photosynthesis and plant growth, phosphorus aerial plant tissue content, and we also calculated indices (ratios) related to leaf growth and above ground tissue water content. Cr(VI) almost exclusively was found in root tissues; all physiological and growth parameters studied were severely affected and plants selectively accumulated phosphorus in aerial plant tissues with increasing Cr(VI) soil concentrations. On the other hand, N amendment resulted in improved plant features in some of the measured parameters: chlorophyll index was improved with added N at T-2, plant height was significantly higher at T-0, T-1, and T-2, and aerial dry weight and leaf area was higher at T-0; these effects indicate that added N did increase P. oleracea potential to ameliorate Cr(VI) toxic effects. We conclude that purslane showed a potential as a possible species to be successfully introduced to Cr(VI)-laden soils, but more research is certainly necessary. © 2021 by the authors.