Basil as secondary crop in cascade hydroponics: Exploring salinity tolerance limits in terms of growth, amino acid profile, and nutrient composition

Abstract

In a cascade hydroponic system, the used nutrient solution drained from a primary crop is directed to a secondary crop, enhancing resource-use efficiency while minimizing waste. Nevertheless, the inevitably increased EC of the drainage solution requires salinity-tolerant crops. The present study explored the salinity-tolerance thresholds of basil to evaluate its potential use as a secondary crop in a cascade system. Two distinct but complemented approaches were used; the first experiment examined basil response to increased levels of salinity (5, 10 and 15 dS m-1, compared with 2 dS m-1 of control) to identify the limits, and the second experiment employed a cascade system with cucumber as a primary crop to monitor basil responses to the drainage solution of 3.2 dS m-1. Growth, ascorbate content, nutrient concentration, and total amino acid concentration and profile were determined in both experiments. Various aspects of basil growth and biochemical performance collectively indicated the 5 dS m??1 salinity level as the upper limit/threshold of tolerance to stress. Higher salinity levels considerably suppressed fresh weight production, though the total concentration of amino acids showed a sevenfold increase under 15 dS m-1 and 4.5-fold under 5 and 10 dS m-1 compared to the control. The performance of basil in the cascade system was subject to a compromise between a reduction of fresh produce and an increase of total amino acids and ascorbate content. This outcome indicated that basil performed well under the conditions and the system employed in the present study, and might be a good candidate for use as a secondary crop in cascade-hydroponics systems. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.