Μελέτη της έκφρασης του γονιδίου της ντεκορίνης σε ισχαιμικό-υποξικό εγκέφαλο επιμυών

Abstract

In the present study we investigate the expression of decorin gene in neonatal rat brain during ichemic and hypoxic conditions. Decorin is a protein molecule which belongs to the family of small rich in leucine proteoglycans (SLRPs) and it is present in several cells through maturation process. Decorin interacts with important factors that influence the cellular cycle as TGF-β (transformer growth factor β) and the receptor of EGF (epidermic growth factor) and with elements of extra cellular matrix as collagen. Those properties of decorin conduct us to the suspicion that it plays an important role in the control of cellular cycle and the process of maturation and proliferation. For the experiment procedure we used seven-day-old wistar rat pups that were exposed in hypoxic conditions (8% oxygen -92% nitrogen mixture for 60 min), two or three hours after permenant occlusion of their left common carotid. We isolated RNA from the brain of rats who were sacrificed at Oh, 2h, 24h, 48h, 72h and 7 days after the ichemic and hypoxic conditions. The RNA was subjected to reserve transcription and competitive PCR amplification under standard conditions including b-actin as internal control for gene expression. Our results demonstrated an initial diminution both in the right and the left hemisphere of decorin gene expression due in the cell death during ichemia / hypoxia and a tension of return in normal levels from 48h. After seven days decorin gene expression seems to increase up to normal levels. This results support previous studies, which correlate the increased levels of decorin during angiogenesis, with the formation of capillaries.