dc.creator | Meletiadis, J. | en |
dc.creator | Antachopoulos, C. | en |
dc.creator | Stergiopoulou, T. | en |
dc.creator | Pournaras, S. | en |
dc.creator | Roilides, E. | en |
dc.creator | Walsh, T. J. | en |
dc.date.accessioned | 2015-11-23T10:39:18Z | |
dc.date.available | 2015-11-23T10:39:18Z | |
dc.date.issued | 2007 | |
dc.identifier | 10.1128/AAC.00345-07 | |
dc.identifier.issn | 664804 | |
dc.identifier.uri | http://hdl.handle.net/11615/30881 | |
dc.description.abstract | Antifungal agents may differ in their fungicidal activities against Aspergillus spp. In order to compare the fungicidal activities of voriconazole and amphotericin B against 40 isolates of Aspergillus fumigatus, A. flavus, and A. terreus, we developed a new microbroth colorimetric method for assessing fungicidal activities and determining minimal fungicidal concentrations (MFCs). This methodology follows the antifungal susceptibility testing reference method M-38A for MIC determination. After drug removal and addition of fresh medium, growth of viable conidia adhering to the bottoms of the microtitration wells was assessed by a colorimetric assay of metabolic activity after 24 h of incubation. The new method was faster (six times), reproducible (92 to 97%), and in agreement with culture-based MFCs (91 to 100%). Differential fungicidal activities of voriconazole and amphotericin B were found among the three Aspergillus species, with A. fumigatus and A. flavus having the lowest (1 and 2 mg/liter, respectively) and A. terreus the highest (>16 mg/liter) median amphotericin B MFCs; A. flavus had a lower median voriconazole MFC (4 mg/liter) than the other species (>8 mg/liter; P < 0.05). Amphotericin B was fungicidal (MFC/MIC ≤ 4) against all A. fumigatus and A. flavus isolates but no A. terreus isolates, whereas voriconazole was fungicidal against 82% of A. flavus isolates and fungistatic (MFC/MIC > 4) against 94% of A. fumigatus and 84% of A. terreus isolates. The new methodology revealed a concentration- dependent sigmoid pattern of fungicidal effects, indicating that fungicidal activity is not an all-or-nothing phenomenon and that some degree of fungicidal action can be found even for agents considered fungistatic based on the MFC/MIC ratio. Copyright © 2007, American Society for Microbiology. All Rights Reserved. | en |
dc.source.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-35948947274&partnerID=40&md5=0d46356a1a9c88f960e5d2201c6c7b18 | |
dc.subject | 2,3 bis(2 methoxy 4 nitro 5 sulfophenyl) 2h tetrazolium 5 carboxanilide | en |
dc.subject | amphotericin B | en |
dc.subject | tetrazolium | en |
dc.subject | unclassified drug | en |
dc.subject | voriconazole | en |
dc.subject | antifungal activity | en |
dc.subject | article | en |
dc.subject | aspergillosis | en |
dc.subject | Aspergillus | en |
dc.subject | Aspergillus flavus | en |
dc.subject | Aspergillus fumigatus | en |
dc.subject | Aspergillus terreus | en |
dc.subject | colorimetry | en |
dc.subject | concentration response | en |
dc.subject | diagnostic value | en |
dc.subject | early diagnosis | en |
dc.subject | fungus isolation | en |
dc.subject | laboratory automation | en |
dc.subject | microbroth methodology | en |
dc.subject | minimum fungicidal concentration | en |
dc.subject | minimum inhibitory concentration | en |
dc.subject | nonhuman | en |
dc.subject | priority journal | en |
dc.subject | reproducibility | en |
dc.subject | species identification | en |
dc.subject | standardization | en |
dc.subject | Antibiotics, Antifungal | en |
dc.subject | Antifungal Agents | en |
dc.subject | Colony Count, Microbial | en |
dc.subject | Culture Media | en |
dc.subject | Microbial Sensitivity Tests | en |
dc.subject | Pyrimidines | en |
dc.subject | Quality Control | en |
dc.subject | Regression Analysis | en |
dc.subject | Reproducibility of Results | en |
dc.subject | Species Specificity | en |
dc.subject | Spores, Fungal | en |
dc.subject | Tetrazolium Salts | en |
dc.subject | Triazoles | en |
dc.subject | Vitamin K 3 | en |
dc.title | Differential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodology | en |
dc.type | journalArticle | en |