Genetic structure of Greek brown hare (Lepus europaeus) populations as revealed by mtDBNA RFLP-PCR analysis: implications for conserving genetic diversity

Abstract

The genetic differentiation and the phylogenetic status of brown hare (Lepus europaeus) populations from central Greece as well as the impact of the releases of reared individuals on the native populations genetic structure was assessed, using mtDNA RFLP-RCR analysis. Data analysis revealed extensive haplotype diversity (42 out of 56 haplotypes were unique) within and among wild populations, Haplotype diversity was equally distributed within and between geographical regions, while significant genetic structuring was evident from heterogeneity of haplotype frequencies among sampling sites. Specific mtDNA profiles clearly differentiated reared from wild individuals and proved highly indicative for reared hares from past releases caught within wild populations. MtDNA analysis suggests the introgression of allochthonous gene pools into the native populations. To conserve indigenous genotypes and to prevent loss of genetic diversity, restocking operations should be stopped and an appropriate management adjusted to the local population dynamics should be developed. (C) 2001 Elsevier Science Ltd. All rights reserved.