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Carbapenemase-producing Pseudomonas aeruginosa from central Greece: molecular epidemiology and genetic analysis of class I integrons
dc.creator | Liakopoulos, A. | en |
dc.creator | Mavroidi, A. | en |
dc.creator | Katsifas, E. A. | en |
dc.creator | Theodosiou, A. | en |
dc.creator | Karagouni, A. D. | en |
dc.creator | Miriagou, V. | en |
dc.creator | Petinaki, E. | en |
dc.date.accessioned | 2015-11-23T10:37:44Z | |
dc.date.available | 2015-11-23T10:37:44Z | |
dc.date.issued | 2013 | |
dc.identifier | 10.1186/1471-2334-13-505 | |
dc.identifier.issn | 1471-2334 | |
dc.identifier.uri | http://hdl.handle.net/11615/30284 | |
dc.description.abstract | Background: Multidrug-resistant Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy of nosocomial infections, as it possesses several mechanisms of antimicrobial resistance. In Central Greece, a sudden increase of infections caused by carbapenem-resistant P. aeruginosa was observed during 2011, indicating the need for further analysis. Methods: Five-hundred and sixty-eight P. aeruginosa isolates were collected consecutively during an 8-month period in 2011 from inpatients treated in three hospitals in the Thessaly region (1,000,000 habitants) of Greece. Carbapenem resistant P. aeruginosa (n = 284) were characterized by antimicrobial susceptibility testing and beta-lactamase content, and the genetic relatedness of carbapenemase-producing isolates was assessed by BOX-PCR, multilocus sequence typing, and eBURST analysis. Mapping of the class I integrons of Verona integron-encoded metallo-beta-lactamase (VIM)-carrying isolates was also performed, and clinical data of the VIM producers were reviewed. Results: Eighty (14.1%) out of the 568 P. aeruginosa isolates recovered from clinical specimens were VIM producers. Multilocus sequence typing revealed high prevalence of the international clones ST111 and ST235 among blaVIM-2- and blaVIM-4-positive isolates, respectively. blaVIM-17 was identified in an isolate of a novel sequence type (ST1457). bla(VIM) gene cassettes were carried by five distinct class I integrons, including two novel ones. Conclusions: Since the first report of VIM-producing P. aeruginosa in 2000, this microorganism still remains among the most prevalent multidrug resistant pathogens in Greece. The spread of VIM-producers belonging to the most common international clones (ST111 and ST235), the spread of integrons of divergent structures, and the emergence of novel integrons underscore their ongoing evolution. | en |
dc.source | Bmc Infectious Diseases | en |
dc.source.uri | <Go to ISI>://WOS:000328903000003 | |
dc.subject | Pseudomonas aeruginosa | en |
dc.subject | MDR | en |
dc.subject | Carbapenems | en |
dc.subject | VIM | en |
dc.subject | Integrons | en |
dc.subject | MLST | en |
dc.subject | METALLO-BETA-LACTAMASE | en |
dc.subject | OUTBREAK | en |
dc.subject | CLONES | en |
dc.subject | RESISTANCE | en |
dc.subject | BLA(VIM-2) | en |
dc.subject | IDENTIFICATION | en |
dc.subject | ACQUISITION | en |
dc.subject | EMERGENCE | en |
dc.subject | STRAINS | en |
dc.subject | VIM-2 | en |
dc.subject | Infectious Diseases | en |
dc.title | Carbapenemase-producing Pseudomonas aeruginosa from central Greece: molecular epidemiology and genetic analysis of class I integrons | en |
dc.type | journalArticle | en |
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