Πλοήγηση ανά Συγγραφέα "Markoulatos, P."
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Enteroviral infection in Greek AIDS patients
Papaventsis, D.; Markoulatos, P.; Mangafas, N.; Lazanas, M.; Levidiotou-Stefanou, S. (2004)Objective: Prolonged intestinal replication of polioviruses has not previously been studied in Greek AIDS patients. The objective of our study was to estimate the prevalence of enteroviral infections in this population. ... -
Environmental surveillance of circulation of enteroviruses-Public health effects
Papaventsis, D.; Siafakas, N.; Markoulatos, P.; Sopidou, V.; Economou, C.; Levidiotou-Stefanou, S. (2006)Objective: This study presents a new approach for the molecular "serotyping" of enteroviruses concentrated and isolated from urban sewage. Method: Samples were collected from the Nicosia Sewage Treatment Plant in Cyprus. ... -
Evaluation of seroneutralization and molecular diagnostic methods for echovirus identification
Kottaridi, C.; Bolanaki, E.; Siafakas, N.; Markoulatos, P. (2005)In this study we compared the identification results of 41 echovirus clinical isolates using RIVM pools (National Institute for Public Health and the Environment RIVM, Bilthoven, The Netherlands) and reverse transcriptio ... -
Evolution of 2B and 2C genomic parts of species B Coxsackie viruses. Phylogenetic study and comparison with other regions
Bolanaki, E.; Kottaridi, C.; Markoulatos, P.; Margaritis, L.; Katsorchis, T. (2006)Modern molecular approaches on the genome of enteroviruses' circulating strains have established new data about the mechanism and significance of its evolution. In the present study, 46 enteroviruses isolates, belonging ... -
Evolution of a rare vaccine-derived multirecombinant poliovirus
Karakasiliotis, I.; Paximadi, E.; Markoulatos, P. (2005)Recombination is one of the mechanisms by which viral genomes evolve. A vaccine-derived multirecombinant poliovirus strain was isolated from a 5-month-old child with vaccine-associated paralytic poliomyelitis after oral ... -
Full-Genome Sequence Analysis of a Multirecombinant Echovirus 3 Strain Isolated from Sewage in Greece
Kyriakopoulou, Z.; Dedepsidis, E.; Pliaka, V.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary ... -
Genome analysis of two type 6 echovirus (E6) strains recovered from sewage specimens in Greece in 2006
Kyriakopoulou, Z.; Pliaka, V.; Tsakogiannis, D.; Ruether, I. G. A.; Komiotis, D.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)Echovirus 6 (E6) is one of the main enteroviral serotypes that was isolated from cases of aseptic meningitis and encephalitis during the last years in Greece. Two E6 (LR51A5 and LR61G3) were isolated from the sewage treatment ... -
Genomic analysis of recombinant Sabin clinical isolates
Paximadi, E.; Karakasiliotis, I.; Mamuris, Z.; Stathopoulos, C.; Krikelis, V.; Markoulatos, P. (2006)Recombination in Poliovirus vaccine strains is a very frequent phenomenon. In this report 23 polio/Sabin strains isolated from healthy vaccinees or from VAPP patients after OPV administration, were investigated in order ... -
Growth kinetic analysis of bi-recombinant poliovirus vaccine strains
Pliaka, V.; Dedepsidis, E.; Kyriakopoulou, Z.; Papadi, G.; Tsakogiannis, D.; Pratti, A.; Levidiotou-Stefanou, S.; Markoulatos, P. (2010)Attenuated strains of Sabin poliovirus vaccine replicate in the human gut and in rare cases may cause vaccine-associated paralytic poliomyelitis (VAPP). Mutations at specific sites of the genome and recombination between ... -
High sequence divergence in the 5 ' non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis
Georgopoulou, A.; Markoulatos, P.; Spyrou, N.; Vakalis, N.; Bei, T. A.; Vamvakopoulos, N. C. (2001)We report the restriction fragment length polymorphism (RFLP) patterns of a 440-bp-long 5' non-coding region (5'NCR) amplification target of all 34 reference Coxsackie B and ECHO (enteric cytopathic human orphan) enterovirus ... -
Identification of novel E6-E7 sequence variants of human papillomavirus 16
Tsakogiannis, D.; Kyriakopoulou, Z.; Amoutzias, G.; Ruether, I. G. A.; Dimitriou, T. G.; Panotopoulou, E.; Markoulatos, P. (2013)The rate of evolution of the human papillomavirus 16 (HPV16) genome is low. However, the ability of the E6 oncoprotein to interact with distinct p53 variants causes selective pressure on the E6 gene. In addition, intratypic ... -
Improved genotyping vaccine and wild-type poliovirus strains by restriction fragment length polymorphism analysis: Clinical diagnostic implications
Georgopoulou, A.; Markoulatos, P.; Spyrou, N.; Vamvakopoulos, N. C. (2000)The combination of preventive vaccination and diagnostic typing of viral isolates from patients with clinical poliomyelitis constitutes our main protective shield against polioviruses. The restriction fragment length ... -
Internal translation initiation stimulates expression of the ARF/core+1 open reading frame of HCV genotype 1b
Boumlic, A.; Vassilaki, N.; Dalagiorgou, G.; Kochlios, E.; Kakkanas, A.; Georgopoulou, U.; Markoulatos, P.; Orfanoudakis, G.; Mavromara, P. (2011)The hepatitis C virus possesses an alternative open reading frame overlapping the Core gene, whose products are referred to as Core+1 or alternative reading frame (ARF) or F protein(s). Extensive studies on genotype HCV-1 ... -
Laboratory diagnosis of contagious ecthyma: Comparison of different PCR protocols with virus isolation in cell culture
Kottaridi, C.; Nomikou, K.; Lelli, R.; Markoulatos, P.; Mangana, O. (2006)A new polymerase chain reaction (PCR) assay for rapid diagnosis of contagious ecthyma was designed and applied to 21 clinical samples from Greece. This assay, which detects a highly conserved gene from the parapox genome, ... -
Membrane adsorption with direct cell culture combined with reverse transcription-PCR as a fast method for identifying enteroviruses from sewage
Papaventsis, D.; Siafakas, N.; Markoulatos, P.; Papageorgiou, G. T.; Kourtis, C.; Chatzichristou, E.; Economou, C.; Levidiotou, S. (2005)We present a new approach for the detection and identification of enteroviruses concentrated and isolated from sewage. Samples were collected from two study sites located at Nicosia and Limassol sewage treatment plants in ... -
Molecular and evolutionary analysis of HPV16 E6 and E7 genes in Greek women
Tsakogiannis, D.; Papadopoulou, A.; Kontostathi, G.; Ruether, I. G. A.; Kyriakopoulou, Z.; Dimitriou, T. G.; Orfanoudakis, G.; Markoulatos, P. (2013)Human papillomavirus type 16 (HPV16) non-European variants have been associated with persistent infection and cervical cancer development, while the L83V variant of the E6 gene has been correlated with the progression of ... -
Molecular and phylogenetic analysis of haemagglutinin and neuraminidase sequences from recent human influenza type A (H3N2) viral isolates in Southern Greece
Plakokefalos, E. T.; Markoulatos, P.; Spyrou, N.; Bei, T. A.; Vamvakopoulos, N. C. (2001)Eighteen haemagglutinin (HAI) gene segments and eleven neuraminidase (NA) genes of human influenza type A (H3N2) viruses isolated from non-vaccinated individuals presenting severe influenza-like illness at peak influenza ... -
Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
Tsakogiannis, D.; Ruether, I. G. A.; Kyriakopoulou, Z.; Pliaka, V.; Skordas, V.; Gartzonika, C.; Levidiotou-Stefanou, S.; Markoulatos, P. (2012)The HPV16 E1(a )E4 protein is thought to contribute to the release of newly formed viral particles from infected epithelia. In order to investigate amino acid mutations in the HPV16 E1(a )E4 protein, the complete E4 ORF ... -
Molecular characterization of a new intergenotype Norovirus GII recombinant
Ruether, I. G. A.; Tsakogiannis, D.; Pliaka, V.; Kyriakopoulou, Z.; Krikelis, A.; Gartzonika, C.; Leveidiotou-Stefanou, S.; Markoulatos, P. (2012)Human noroviruses (NoVs) of the Caliciviridae family are a major cause of epidemic gastroenteritis. The NoV genus is genetically diverse and recombination of viral RNA is known to depend upon various immunological and ... -
Molecular characterization of wild-type polioviruses isolated in Greece during the 1996 outbreak in Albania
Kyriakopoulou, Z.; Kottaridi, C.; Dedepsidis, E.; Bolanaki, E.; Levidiotou-Stefanou, S.; Markoulatos, P. (2006)During the present study three type 1 poliovirus strains isolated in Greece during the 1996 poliomyelitis outbreak in Albania were retrospectively investigated and determination of their relationship with other epidemic ...