Insecticide resistance status of the codling moth Cydia pomonella (Lepidoptera: Tortricidae) from Greece
Συγγραφέας
Voudouris, C. C.; Sauphanor, B.; Franck, P.; Reyes, M.; Mamuris, Z.; Tsitsipis, J. A.; Vontas, J.; Margaritopoulos, J. T.Ημερομηνία
2011Λέξη-κλειδί
Επιτομή
The codling moth Cydia pomonella L is controlled mostly with chemical insecticides in Greece and control failures have been reported. However, there are no insecticide resistance studies in the country as yet. We examined the insecticide resistance status of 33 and 38 populations of fifth-instar non-diapausing and diapausing larvae, respectively by applying bioassays, biochemical and DNA diagnostics. Diagnostic concentrations of azinphos-methyl, phosalone, deltamethrin, thiacloprid, fenoxycarb, tebufenozide, methoxyfenozide and diflubenzuron were used in bioassays. Almost all populations showed reduced susceptibility to at least one insecticide and approximately half of them to all insecticides examined compared to a laboratory susceptible strain used as reference. However, only one out of six populations tested showed reduced susceptibility in ovicidal tests with fenoxycarb. Cross-resistances were observed among most insecticides, except from the pairs fenoxycarb-phosalone and thiacloprid-phosalone, in non-diapausing larvae. The more obvious biochemical marker associated with the reduced susceptibility observed in both larval instars was elevated cytochrome P-450 Polysubstrate monooxygenases activity, followed by elevated glutathione-S-transferase activity and reduced carboxylesterases activity. Neither sodium channel nor AChE known resistance mutations were found in any of the approximately 1000 individuals of each larval instar screened with diagnostic PCR. Actions for Integrated Resistance Management and application of alternative control methods are discussed. (C) 2011 Elsevier Inc. All rights reserved.
Collections
Related items
Showing items related by title, author, creator and subject.
-
Inactivation, removal, and regrowth potential of opportunistic pathogens and antimicrobial resistance genes in recycled water systems
Drigo B., Brunetti G., Aleer S.C., Bell J.M., Short M.D., Vasileiadis S., Turnidge J., Monis P., Cunliffe D., Donner E. (2021)With two thirds of the global population living in areas affected by water scarcity, wastewater reuse is actively being implemented or explored by many nations. There is a need to better understand the efficacy of recycled ... -
Shotgun metagenomics assessment of the resistome, mobilome, pathogen dynamics and their ecological control modes in full-scale urban wastewater treatment plants
Karaolia P., Vasileiadis S., G. Michael S., G. Karpouzas D., Fatta-Kassinos D. (2021)The conventional activated sludge (CAS) process has limited capacity to remove pathogenic microorganisms and antibiotic resistance genes (ARGs), compared to membrane bioreactors (MBRs). However, the full extent of pathogenic ... -
Low-Grade Systemic Inflammation Interferes with Anabolic and Catabolic Characteristics of the Aged Human Skeletal Muscle
Draganidis D., Jamurtas A.Z., Chondrogianni N., Mastorakos G., Jung T., Grune T., Papadopoulos C., Papanikolaou K., Papassotiriou I., Papaevgeniou N., Poulios A., Batrakoulis A., Deli C.K., Georgakouli K., Chatzinikolaou A., Karagounis L.G., Fatouros I.G. (2021)Aging is associated with the development of chronic low-grade systemic inflammation (LGSI) characterized by increased circulating levels of proinflammatory cytokines and acute phase proteins such as C-reactive protein ...