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dc.creatorValasi, I.en
dc.creatorTheodosiadou, E.en
dc.creatorFthenakis, G. C.en
dc.creatorPapanikolaou, T.en
dc.creatorDeligiannis, C.en
dc.creatorKalogiannis, D.en
dc.creatorChadio, S.en
dc.creatorAmiridis, G. S.en
dc.date.accessioned2015-11-23T10:53:05Z
dc.date.available2015-11-23T10:53:05Z
dc.date.issued2013
dc.identifier10.1016/j.anireprosci.2013.02.026
dc.identifier.issn0378-4320
dc.identifier.urihttp://hdl.handle.net/11615/34239
dc.description.abstractBlood concentrations of progesterone, FSH and oestradiol in Karagouniko ewes subjected to laparoscopic ovum pick-up (OPU) at specific stages of induced oestrous cycle, were measured. Twenty-four cyclic ewes were randomly allocated into four equal groups (A, B, C and D). Oestrus was synchronized with progestagen intravaginal sponges and detected by teaser rams (oestrus: day 0). In group A, during the induced oestrous cycle, OPU was performed on days 4,9 and 14 (sessions A1, A2 and A3, respectively). In group B and group D, OPU was performed once, on day 9 and 14, respectively. In group C (controls), endoscopic observation of follicular population was performed three times, as in group A. Starting at sponge removal, progesterone was measured in blood samples collected on 22 daily occasions and oestradiol in samples collected on 27 occasions collected at various time-points starting 2 h before to 24 h after OPU. Follicular populations did not differ among A1, A2, A3 or between C1, C2, C3 and A1, A2, A3 or A1, B, D, respectively. Oocytes of better quality (category '1' or '2') were collected at A3 session compared with A1 (P < 0.05). Progesterone concentration and oestrous cycle length did not differ among groups. Decreased oestradiol concentrations followed by FSH increase were recorded 3-5 h after OPU. The results confirm the regulatory role of oestradiol on FSH secretion. The quality of collected oocytes was improved in subsequent pick-up sessions in the oestrous cycle. Moreover, OPU at specific stages of the luteal phase of the cycle, even when applied repeatedly, do not affect the normal oestrous cycle length of ewes. (C) 2013 Elsevier B.V. All rights reserved.en
dc.source.uri<Go to ISI>://WOS:000319183300008
dc.subjectEwesen
dc.subjectFollicular aspirationen
dc.subjectFSHen
dc.subjectOestradiolen
dc.subjectProgesteroneen
dc.subjectOestrousen
dc.subjectcycleen
dc.subjectESTROUS-CYCLEen
dc.subjectIN-VITROen
dc.subjectOVARIAN-FOLLICLESen
dc.subjectCORPUS-LUTEUMen
dc.subjectASPIRATIONen
dc.subjectSHEEPen
dc.subjectGROWTHen
dc.subjectONSETen
dc.subjectDYNAMICSen
dc.subjectAgriculture, Dairy & Animal Scienceen
dc.subjectReproductive Biologyen
dc.titleEndocrinological profile and follicular development in cyclic ewes subjected to repeated ovum pick-upen
dc.typejournalArticleen


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