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dc.creatorTsamandas, A. C.en
dc.creatorAntonacopoulou, A.en
dc.creatorKalogeropoulou, C.en
dc.creatorTsota, I.en
dc.creatorZabakis, P.en
dc.creatorGiannopoulou, E.en
dc.creatorLiava, A.en
dc.creatorTzelepi, V.en
dc.creatorTepetes, K.en
dc.creatorPetsas, T.en
dc.creatorKardamakis, D.en
dc.creatorKalofonos, H. P.en
dc.date.accessioned2015-11-23T10:50:47Z
dc.date.available2015-11-23T10:50:47Z
dc.date.issued2007
dc.identifier10.1111/j.1872-034X.2007.00124.x
dc.identifier.issn1386-6346
dc.identifier.urihttp://hdl.handle.net/11615/33789
dc.description.abstractOval cells are liver stem cells involved in liver regeneration following liver damage. Previous studies have shown that pretreatment with a hepatocyte inhibitor is required to allow full oval cell activation. This study investigates whether oval cells develop and proliferate in a model of experimental liver fibrosis without pretreatment with a known hepatocyte inhibitor. The study comprised 66 male Wistar rats divided into two groups: A (n = 6): controls; and B (n = 60): CCl4 injection (intraperitoneally 2 mL/kg bodyweight 1:1 volume in corn oil twice weekly). Rats were sacrificed at four, eight and 12 weeks. Liver tissues were evaluated for the degree of fibrosis (Masson's trichrome), cell proliferation (Ki67 antigen), expression of alpha-fetoprotein (AFP) mRNA (RT-PCR and in situ hybridization), AFP protein (Western blot) and cytokeratin-19. Cells with morphologic features of oval cells that were cytokeratin 19 (CK19)+ and AFP mRNA+ were scored in morphometric analysis. Oval cells were present in all 66 specimens; their percentage was higher in group B compared to group A (P < 0.001). AFP mRNA and protein expression increased as fibrosis advanced. Similarly, the numbers of CK19+, AFP mRNA+ and Ki67+ oval cells were higher in advanced fibrosis stages. This study demonstrates that oval cells develop and proliferate in a model of experimental liver fibrosis without pretreatment with a known hepatocytic inhibitor. However, further research is warranted in order to identify the exact molecular mechanisms involved in this process.en
dc.source.uri<Go to ISI>://WOS:000247806000011
dc.subjectcarbon tetrachlorideen
dc.subjectcirrhosisen
dc.subjectliver fibrosisen
dc.subjectoval cellsen
dc.subjectratsen
dc.subjectRT-PCRen
dc.subjectCHOLINE-DEFICIENT DIETen
dc.subjectCHRONIC HEPATITIS-Cen
dc.subjectLIVER-REGENERATIONen
dc.subjectSTEM-CELLen
dc.subjectPROGENITOR CELLSen
dc.subjectD-GALACTOSAMINEen
dc.subjectEXPRESSIONen
dc.subjectDIFFERENTIATIONen
dc.subjectHEPATOCYTESen
dc.subjectACTIVATIONen
dc.subjectGastroenterology & Hepatologyen
dc.titleOval cell proliferation in cirrhosis in rats. An experimental studyen
dc.typejournalArticleen


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