Differential regulation of chemokine CCL5 expression in monocytes/macrophages and renal cells by Y-box protein-1
AuthorRaffetseder, U.; Rauen, T.; Djudjaj, S.; Kretzler, M.; En-Nia, A.; Tacke, F.; Zimmermann, H. W.; Nelson, P. J.; Frye, B. C.; Floege, J.; Stefanidis, I.; Weber, C.; Mertens, P. R.
The Y-box protein-1 (YB-1) belongs to the family of cold shock proteins that have pleiotropic functions such as gene transcription, RNA splicing, and mRNA translation. YB-1 has a critical role in atherogenesis due to its regulatory effects on chemokine CCL5 (RANTES) gene transcription in vascular smooth muscle cells. Since CCL5 is a key mediator of kidney transplant rejection, we determined whether YB-1 is involved in allograft rejection by manipulating its expression. In human kidney biopsies, YB-1 transcripts were amplified 17-fold in acute and 21-fold in chronic allograft rejection with a close correlation between CCL5 and YB-1 mRNA expression in both conditions. Among three possible YB-1 binding sites in the CCL5 promoter, a critical element was mapped at -28/-10 bps. This site allowed up-regulation of CCL5 transcription in monocytic THP-1 and HUT78 T-cells and in human primary monocytes; however, it repressed transcription in differentiated macrophages. Conversely, YB-1 knockdown led to decreased CCL5 transcription and secretion in monocytic cells. We show that YB-1 is a cell-type specific regulator of CCL5 expression in infiltrating T-cells and monocytes/ macrophages and acts as an adaptive controller of inflammation during kidney allograft rejection.