Exercise as a model to study redox homeostasis in blood: the effect of protocol and sampling point
AuthorNikolaidis, M. G.; Kyparos, A.; Dipla, K.; Zafeiridis, A.; Sambanis, M.; Grivas, G. V.; Paschalis, V.; Theodorou, A. A.; Papadopoulos, S.; Spanou, C.; Vrabas, I. S.
Twenty males ran either on a level treadmill (nonmuscle-damaging condition) or on a downhill treadmill (muscle-damaging condition). Blood and urine samples were collected before and after exercise (immediately after, 1h, 4h, 24h, 48h, and 96h). The following assays were performed: F-2-isoprostanes in urine, protein carbonyls in plasma, glutathione, superoxide dismutase, glutathione peroxidase, and catalase in erythrocytes. The main finding was that monophasic redox responses were detected after nonmuscle-damaging exercise compared to the biphasic responses detected after muscle-damaging exercise. Based on these findings, muscle-damaging exercise may be a more appropriate experimental model to induce physiological oxidative stress.