beta-Glucocerebrosidase gene mutations in two cohorts of Greek patients with sporadic Parkinson's disease
AuthorMoraitou, M.; Hadjigeorgiou, G.; Monopolis, I.; Dardiotis, E.; Bozi, M.; Vassilatis, D.; Vilageliu, L.; Grinberg, D.; Xiromerisiou, G.; Stefanis, L.; Michelakakis, H.
An increasing number of clinical, neuropathological and experimental evidence linking Gaucher disease and a spectrum of synucleinopathies, including Parkinson's disease (PD) has emerged over the last decade. In particular, several studies, despite individual differences, have shown that mutations in the beta-glucocerebrosidase gene (GBA) are a risk factor for PD. Recently a study from Northern Greece has shown a significant overrepresentation of such mutations only in patients with early onset PD. In the present study 8 different GBA mutations covering 87% of the mutations identified in Gaucher disease patients diagnosed in Greece were investigated in two ethnic Greek cohorts of patients with sporadic Parkinson's disease. Cohort A included patients residing and originating from Thessaly, Central Greece (n = 100) and cohort B included patients residing and/or originating from the greater area of Athens (n = 105). Age-gender-ethnicity matched healthy individuals from the same areas were included as controls (n = 206). In patients of cohort A 11 carriers of GBA mutations were identified (5/11:N3705, 2/11:L444P, 2/11: D409H;H255Q 1/11:H255Q 1/11D409H) as opposed to 3 in the controls (n = 105) (1/3:N370S, 1/3:H255Q 1/3:Y108C) (p=0.021, OR 4.2, 95% CI = 1.14-15.54). In patients of cohort B 10 carriers of GBA mutations were identified (4/10:L444P, 4/10:D409H;H255Q 1/10:N370S, 1/10: IVS10-1G -> A) as opposed to 4 in controls (11 = 101) (3/4:N370S, 1/4:L444P). However the difference was not statistically significant (p = 0.113, OR 2.5,95% CI = 0.77-8.42). In both cohorts, patients with PD harboring a GBA mutation had an earlier onset of symptoms than non-carriers (p = 0.034, p = 0.004). The overall difference in the number of carriers identified in PD patients and controls was statistically significant (p = 0.006; OR 3.24; 95% CI = 1.35-7.81). The association was reinforced in the early onset PD patients (EOPD; n = 28, p = 0.000, OR11.37; 95% CI = 3.73-34.6). In conclusion GBA mutations were identified with increased frequency in both geographical cohorts of patients with sporadic PD studied compared to control individuals, with the difference being statistically significant only in cohort A. An impressive association with EOPD was found and one third of the EOPD patients examined harbored a GBA mutation. Qualitative differences regarding the type of mutations and/or their relative frequencies were observed between cohorts A and B of PD patients. Genetic and/or environmental factors may account for the observed differences. (C) 2011 Elsevier Inc. All rights reserved.