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dc.creatorMeletiadis, J.en
dc.creatorAntachopoulos, C.en
dc.creatorStergiopoulou, T.en
dc.creatorPournaras, S.en
dc.creatorRoilides, E.en
dc.creatorWalsh, T. J.en
dc.description.abstractAntifungal agents may differ in their fungicidal activities against Aspergillus spp. In order to compare the fungicidal activities of voriconazole and amphotericin B against 40 isolates of Aspergillus fumigatus, A. flavus, and A. terreus, we developed a new microbroth colorimetric method for assessing fungicidal activities and determining minimal fungicidal concentrations (MFCs). This methodology follows the antifungal susceptibility testing reference method M-38A for MIC determination. After drug removal and addition of fresh medium, growth of viable conidia adhering to the bottoms of the microtitration wells was assessed by a colorimetric assay of metabolic activity after 24 h of incubation. The new method was faster (six times), reproducible (92 to 97%), and in agreement with culture-based MFCs (91 to 100%). Differential fungicidal activities of voriconazole and amphotericin B were found among the three Aspergillus species, with A. fumigatus and A. flavus having the lowest (1 and 2 mg/liter, respectively) and A. terreus the highest (>16 mg/liter) median amphotericin B MFCs; A. flavus had a lower median voriconazole MFC (4 mg/liter) than the other species (>8 mg/liter; P < 0.05). Amphotericin B was fungicidal (MFC/MIC ≤ 4) against all A. fumigatus and A. flavus isolates but no A. terreus isolates, whereas voriconazole was fungicidal against 82% of A. flavus isolates and fungistatic (MFC/MIC > 4) against 94% of A. fumigatus and 84% of A. terreus isolates. The new methodology revealed a concentration- dependent sigmoid pattern of fungicidal effects, indicating that fungicidal activity is not an all-or-nothing phenomenon and that some degree of fungicidal action can be found even for agents considered fungistatic based on the MFC/MIC ratio. Copyright © 2007, American Society for Microbiology. All Rights Reserved.en
dc.subject2,3 bis(2 methoxy 4 nitro 5 sulfophenyl) 2h tetrazolium 5 carboxanilideen
dc.subjectamphotericin Ben
dc.subjectunclassified drugen
dc.subjectantifungal activityen
dc.subjectAspergillus flavusen
dc.subjectAspergillus fumigatusen
dc.subjectAspergillus terreusen
dc.subjectconcentration responseen
dc.subjectdiagnostic valueen
dc.subjectearly diagnosisen
dc.subjectfungus isolationen
dc.subjectlaboratory automationen
dc.subjectmicrobroth methodologyen
dc.subjectminimum fungicidal concentrationen
dc.subjectminimum inhibitory concentrationen
dc.subjectpriority journalen
dc.subjectspecies identificationen
dc.subjectAntibiotics, Antifungalen
dc.subjectAntifungal Agentsen
dc.subjectColony Count, Microbialen
dc.subjectCulture Mediaen
dc.subjectMicrobial Sensitivity Testsen
dc.subjectQuality Controlen
dc.subjectRegression Analysisen
dc.subjectReproducibility of Resultsen
dc.subjectSpecies Specificityen
dc.subjectSpores, Fungalen
dc.subjectTetrazolium Saltsen
dc.subjectVitamin K 3en
dc.titleDifferential fungicidal activities of amphotericin B and voriconazole against Aspergillus species determined by microbroth methodologyen

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