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dc.creatorManiati, M.en
dc.creatorIkonomidis, A.en
dc.creatorMantzana, P.en
dc.creatorDaponte, A.en
dc.creatorManiatis, A. N.en
dc.creatorPournaras, S.en
dc.description.abstractObjectives: A Pseudomonas aeruginosa clinical isolate that exhibited high-level carbapenem resistance and produced metallo-beta-lactamase (MBL) was recovered from a Greek patient. This study was conducted to determine the underlying mechanisms that conferred the carbapenem resistance phenotype. Methods: MICs were determined by Etest and Etest MBL. PCR assays were performed for identification of bla(VIM-type), other antibiotic resistance and efflux pump genes and mapping of class 1 integrons. Expression of efflux pump genes was quantified by real-time PCR. Nucleotide sequencing was used to determine the bla(VIM) allele. The location of the MBL allele was investigated by mating experiments, plasmid analysis and hybridization studies. Results: The isolate was highly carbapenem-resistant (MICs of imipenem and meropenern were 512 and 128 mg/L, respectively) and multidrug-resistant. It harboured the P-lactamase genes bla(VIM-4) and bla(P1b) in a novel class 1 integron named InV4P1, and a second integron with aac(6')-lb and bla(OXA-35) gene cassettes. The isolate was deficient in porin OprD and overexpressed eff lux pumps MexAB-OprM and MexXY-OprM. Conjugation experiments failed to detect transferable MBL determinants, plasmids were not visualized and bla(VIM) was detected by PCR in the chromosomal band. Conclusions: Multiple carbapenem resistance mechanisms are demonstrated to coexist in a single A aeruginosa isolate and might confer the high-level carbapenem resistance.en
dc.sourceJournal of Antimicrobial Chemotherapyen
dc.source.uri<Go to ISI>://WOS:000248180200018
dc.subjectP. aeruginosaen
dc.subjectInfectious Diseasesen
dc.subjectPharmacology & Pharmacyen
dc.titleA highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel bla(VIM-4)/bla(P1b) integron overexpresses two efflux pumps and lacks OprDen

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