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dc.creatorKhalifa, T.en
dc.creatorLymberopoulos, A.en
dc.creatorTheodosiadou, E.en
dc.date.accessioned2015-11-23T10:34:47Z
dc.date.available2015-11-23T10:34:47Z
dc.date.issued2013
dc.identifier10.1016/j.theriogenology.2012.11.009
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11615/29407
dc.description.abstractTwo consecutive randomized double-blind field fertility experiments were conducted over a 4-month period and aimed at evaluating the association of two commercial soybean lecithin-based extenders (AndroMed [Minitub, Tiefenbach, Germany] and BioXcell [IMV Technologies, L'Aigle, France]) with pregnancy rates of chilled-stored (CS) and frozen-thawed (FT) ram semen. Semen samples with more than 2 x 10(9) sperm per mL and 70% progressive motile spermatozoa were collected via an artificial vagina from twelve proven fertile Chios rams, split-diluted with the above mentioned extenders, packaged in 0.25 mL straws and either stored at 5 +/- 1 degrees C for 30 to 36 hours or frozen and thawed. Non-lactating multiparous ewes were inseminated in progestagen-synchronized estrus either with CS (AndroMed: N = 212 and BioXcell: N = 206; intracervical AI) or with FT (AndroMed: N = 114 and BioXcell: N = 92; laparoscopic intrauterine AI) semen. Ovulation was confirmed in all ewes based on determination of blood plasma progesterone (>1 ng/mL) 8 days post AI. Ewes were screened for pregnancy diagnosis by transabdominal ultrasonography 65 days post AI. BioXcell was superior to AndroMed in preserving the fertilizing potential of CS (P < 0.05) and FT (P < 0.005) semen. In AndroMed-stored semen, young rams (1.5-2.5 years old, N = 8) had a pregnancy rate (59.1%; 124/210) lower than that (72.4%; 84/116) of mature rams (4.5 to 5.5 years, N = 4; P < 0.025). Compared with AndroMed extender, processing of young ram semen in BioXcell extender improved pregnancy rates of CS (66.7%; 88/132 vs. 83.9%; 94/112; P < 0.005) and FT (46.2%; 36/78 vs. 71.0%; 44/62; P < 0.01) spermatozoa. Both extenders were similarly effective in preserving pregnancy rates of mature ram semen (P > 0.05). Ram-by-extender interactions were significant for pregnancy rates of CS and FT semen. Irrespective of extenders, overall pregnancy rates after intracervical and intrauterine AI were 75.1% and 62.2%, respectively (P < 0.001). In conclusion, BioXcell is a suitable extender for short- and long-term storage of ram semen. Selection of the ewes, farms, and extenders for intracervical AI programs can contribute to satisfactory fertility rates with semen preserved more than 24 hours at 5 degrees C. (c) 2013 Elsevier Inc. All rights reserved.en
dc.sourceTheriogenologyen
dc.source.uri<Go to ISI>://WOS:000313933800015
dc.subjectSheepen
dc.subjectSpermatozoaen
dc.subjectAndroMeden
dc.subjectBioXcellen
dc.subjectArtificial inseminationen
dc.subjectPregnancy rateen
dc.subjectFROZEN-THAWED SEMENen
dc.subjectLAPAROSCOPIC ARTIFICIAL-INSEMINATIONen
dc.subjectIN-VITROen
dc.subjectFERTILIZATIONen
dc.subjectBOVINE SERUM-ALBUMINen
dc.subjectCERVICAL INSEMINATIONen
dc.subjectINTRAUTERINEen
dc.subjectINSEMINATIONen
dc.subjectSEASONAL-VARIATIONen
dc.subjectSEMINAL PLASMAen
dc.subjectEGG-YOLKen
dc.subjectOVULATORYen
dc.subjectACTIVITYen
dc.subjectReproductive Biologyen
dc.subjectVeterinary Sciencesen
dc.titleAssociation of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: A randomized double-blind parallel group designen
dc.typejournalArticleen


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