Outbreaks in distinct regions due to a single Klebsiella pneumoniae clone carrying a bla(VIM-1) metallo-beta-lactamase gene
AuthorIkonomidis, A.; Tokatlidou, D.; Kristo, I.; Sofianou, D.; Tsakris, A.; Mantzana, P.; Pournaras, S.; Maniatis, A. N.
From December 2004 to March 2005, 27 Klebsiella pneumoniae clinical isolates that were positive by the imipenem-EDTA double-disk synergy test and that exhibited a single macrorestriction pattern were recovered in two distinct Greek hospitals. The isolates carried a transferable bla(VIM-t) metallo-beta-lactamase gene in a class I integron. Reverse transcriptase PCR showed that the gene was similarly expressed in low- and high-level carbapenem-resistant isolates, indicating the existence of additional resistance mechanisms. The clonal spread of VIM-1-producing K. pneumoniae strains in distinct regions where up to now bla(VIM-2) and bla(VIM-4) alleles were common is worrisome.