Effects of grape extracts on the in vitro activity of enzymes involved in oxidative stress regulation
AuthorChrysoula, S.; Veskoukis, A. S.; Dimitrios, S.; Kalliopi, L.; Maria, A.; Haroutounian, S. A.; Maria, T.; Eleni, T.; Dimitrios, K.
Background/Aim: A number of in vitro and in vivo studies have investigated the potential preventive activity of grape extracts against different diseases, and have mostly focused on their antioxidant properties. The present study examined the effects of 21 extracts from stem, skin and berry from Greek grape varieties on the activity of enzymes involved in regulation of oxidative stress, namely xanthine oxidase (XO), catalase (CAT) and superoxide dismutase (SOD). Materials and Methods: The effects of the extracts on the enzymatic activity of XO, CAT and SOD were studied spectrophotometrically. Results: The tested extracts inhibited CAT and XO activity, while higher extract concentrations are required to induce SOD. However, stem and skin extracts exhibited a different inhibitory pattern against CAT and XO compared to berry extracts. The observed differences are possibly attributed to the extract polyphenolic composition. Conclusion: Although the induction of SOD activity suggests an antioxidant capacity, the inhibition of CAT and XO indicates a pro-oxidant action. In general, the extracts showed pro-oxidant activity, possibly dependent on both their polyphenolic composition and concentration.
Showing items related by title, author, creator and subject.
4-Arylazo-3,5-diamino-1H-pyrazole CDK inhibitors: SAR study, crystal structure in complex with CDK2, selectivity, and cellular effects Kryštof, V.; Cankař, P.; Fryšová, I.; Slouka, J.; Kontopidis, G.; Džubák, P.; Hajdúch, M.; Srovnal, J.; De Azevedo Jr, W. F.; Orság, M.; Paprskářová, M.; Rolčík, J.; Látr, A.; Fischer, P. M.; Strnad, M. (2006)In a routine screening of our small-molecule compound collection we recently identified 4-arylazo-3,5-diamino-1H-pyrazoles as a novel group of ATP antagonists with moderate potency against CDK2-cyclin E. A preliminary SAR ...
Urate crystals induce NLRP3 inflammasome-dependent IL-1β secretion and proliferation in isolated primary human T-cells Eleftheriadis, T.; Pissas, G.; Antoniadi, G.; Makri, P.; Liakopoulos, V.; Stefanidis, I. (2015)Background: Urate through NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome-dependent caspase-1 activation stimulates macrophages to secrete inteleukin-1β (IL-1β). Urate also enhances adaptive immunity ...
Begum, J.; Skamnaki, V. T.; Moffatt, C.; Bischler, N.; Sarrou, J.; Skaltsounis, A. L.; Leonidas, D. D.; Oikonomakos, N. G.; Hayes, J. M. (2015)Phosphorylase kinase (PhK) has been linked with a number of conditions such as glycogen storage diseases, psoriasis, type 2 diabetes and more recently, cancer (Camus et al., 2012 ). However, with few reported structural ...