Μελέτη μέσω RT-PCR της γονιδιακής έκφρασης της ντεκορίνης σε υποξικοισχαιμικό εγκέφαλο νεογέννητων επιμυών
Προβολή/ Άνοιγμα
Συγγραφέας
Τσιαμήτρος, ΗλίαςΌνομα Επιβλέποντος
Μπονάνου-Τζεδάκη, Σοφία
Ημερομηνία
2002Γλώσσα
el
Πρόσβαση
ελεύθερη
Επιτομή
Under hypoxic-ischemic conditions, increased deposition of extracellular matrix
proteins occurs in various tissues. The aim of this study is to investigate the effect of
hypoxia-ischemia on the decorin gene expression in neonatal rat brain.
Seven day old wistar rat pups became ischemic by permanent ligation of the left
common carotid. Hypoxia was induced by exposing the pups in a 8% oxygen - 92%
nitrogen gas mixture for 60 minutes. At the end of this period and after 2 and 24 hours
the animals were decapitated and each cerebral hemisphere was preserved.
Total RNA was extracted from cerebral cortex and hippocampus of both cerebral
hemispheres. Ten μΐ of total RNA were subjected to reverse transcription using the
M-MuLV reverse transcriptase. Five μΐ of the reverse transcription reaction mixture
were subjected to competitive PCR amplification under standard contitions, including
beta-actin as internal control for gene expression.
Decorin mRNA is expressed at comparable levels both in the cerebral cortex and the
hippocampus of the neonatal rat brain. Decorin mRNA levels are of limited abudance
compared to those of beta-actin. Decorin mRNA is expressed at comparable levels
both in the right (hypoxic) and the left (hypoxic - ischemic ) cerebral hemispheres of
the neonatal rat brain.
Our results suggest that hypoxia - ischemia slightly reduced the levels of decorin
mRNA in both cerebral cortex and hippocampus of neonatal rat brain the first 2 hours
after , but within 24 hours this effect seemed to be overturned.
Ακαδημαϊκός Εκδότης
Πανεπιστήμιο Θεσσαλίας. Πρόγραμμα Σπουδών Επιλογής. Τμήμα Ιατρικής Βιοχημείας.